Our Tools
Experimental Approaches:
Molecular Biology techniques for membrane protein sample preparation: Plasmid preparation, Gene cloning, Site-directed mutagenesis, Polymerase chain reaction (PCR), SDS-PAGE, E.Coli.Bacterial Transformation, Protein Overexpression, Protein Extraction and Purification, Deuterium Isotopic Labeling, Spin Labeling (mono-functional spin labeling, bi-functional spin labeling), Protein Reconstitutions into Lipid Bilayered Vesicles, Bicelles, Lipodisq Nanoparticles/Styrene Maliec Acid Lipid Particles (SMALPs).
Advanced Magnetic Resonance techniques: CW-EPR Spectral Measurements, CW-EPR Power Saturation, Double Electron Electron Resonance (DEER), Electron Spin Echo Envelope Modulation (ESEEM), CW-dipolar Broadening EPR, Dynamic Light Scattering (DLS), Circular Dichroism (CD) Spectroscopy, and Solid State NMR for the study of biological systems.
Computational Approaches:
Computational techniques: Molecular Dynamics Simulation for molecular modeling of Membrane Proteins.
Protein-Protein docking for studying KCNE3 and KCNQ1 interactions.
Simulated Annealing Molecular Dynamics Simulations for 3D-structural refinement of membrane proteins.
